Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

R&D Systems | Catalog # 8897-LR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera (8897-LR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001008316

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Lymphotoxin beta R/TNFRSF3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Lymphotoxin beta R/TNFRSF3 protein
Rat Lymphotoxin beta R
(Gln29-Met222)
Accession # NP_001008316		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln29 predicted

Predicted Molecular Mass

49 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to inhibit Lymphotoxin alpha 1/ beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756.
The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation, and Storage

8897-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin  beta R (LT beta R; also called TNFRSF3, TNF RIII,or TNF Rrp) is a type I transmembrane glycoprotein within the TNF receptor superfamily (1-3). The rat LT beta R cDNA encodes a 415 amino acid (aa) protein that includes a 30 aa signal peptide, a 191 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine-rich motifs characteristic of the TNF receptor superfamily (2). Within the ECD, rat LT beta R shares 90% and 66% aa sequence identity with the ECD of mouse and human LT beta R, respectively. Soluble LT beta R, which can be formed by proteolytic cleavage of the ECD, functions as a decoy receptor for LT beta R ligands and is involved in the suppression of autoimmunity (4, 5). LT beta R is constitutively expressed in cells of myeloid lineage and is upregulated during tissue regeneration (6). It is expressed on mesenchymal stromal organizing cells that give rise to primary, secondary, and tertiary lymphoid structures (7-9). Mice deficient in LT beta R fail to form secondary lymphoid structures (4). LT beta R ligands include homotrimers of LIGHT/TNFSF14 and the heterotrimeric Lymphotoxin alpha 1/ beta 2 (2). Ligand engagement of LT beta R has been shown to induce NF kappa B activation through canonical (IKK) or alternative (NIK/RelB) signaling pathways (5, 10). LT beta R signaling induces production of cytokines (TRANCE/RANK Ligand/TNFSF11, IL-7), chemokines (CXCL8/IL-8, CXCL13/BCL/BCA-1, CCL19/MIP-3 beta, CCL21/6Ckine, CCL2/MCP-1), and adhesion molecules (VCAM-1/CD106, ICAM-1/CD54, MAdCAM) (8, 11). LT beta R is involved in lipid metabolism, atherosclerosis, and intestinal epithelial homeostasis (4, 12, 13). It also regulates cell growth and can initiate inflammation-related carcinogenesis (5, 14).

References

  1. Force, W.R. et al. (1995) J. Immunol. 155:5280.
  2. Remouchamps, C. et al. (2011) Cytokine Growth Factor Rev. 22:301.
  3. Crowe, P.D. et al. (1994) Science 264:707.
  4. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
  5. Wolf, M.J. et al. (2010) Oncogene 29:5006.
  6. Ware, C.F. (2005) Annu. Rev. Immunol. 23:787.
  7. Boulianne, B. et al. (2012) Front. Immunol. 3:243.
  8. Mouri, Y. et al. (2011) J. Immunol. 186:5047.
  9. van de Pavert, S.A. and R.E. Mebius (2010) Nat. Rev. Immunol. 10:664.
  10. Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
  11. Mikami, Y. et al. (2014) PLoS One 9:e114791.
  12. Macho-Fernandez, E. et al. (2015) Mucosal Immunol. 8:403.
  13. Browning, J.L. (2012) Mucosal Immunol. 5:228.
  14. Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

NP_001008316

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

For research use only

Customer Reviews for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

There are currently no reviews for this product. Be the first to review Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF and earn rewards!

Have you used Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes