Recombinant Rat NKp30 Fc Chimera Protein, CF Summary
Accession # NP_861543.2
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MES and NaCl.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Natural killer cell p30-related protein (NKp30), also known as Natural cytotoxicity triggering receptor 3 (NCR3), is a type I transmembrane glycoprotein consisting of an extracellular domain (ECD) containing a single V-like immunoglobulin domain, a transmembrane domain containing a positively-charged amino acid (aa) residue, and a short cytoplasmic tail (1). NKp30, along with NKp44/NCR2 and NKp46/NCR1, is a member of Natural Cytotoxicity Receptor (NCR) group of proteins, which play a major role in triggering NK-mediated lysis of tumor cells (2). Although a pseudogene in mice, the ECD of mature rat NKp30 shares 68% aa identity with human NKp30.
In rats, NKp30 is expressed on a subset of NK cells present in blood, spleen, and, to a lesser extent, liver. These tissues also express different percentages and levels of NKp30, which is a departure from the other NK activation receptors which are expressed constitutively (3). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (1, 5). NKp30 has been found to bind to the human protein B7-H6 (6). B7-H6 binding activates the NKp30 pathway leading to cytokine release and cytotoxic function against target cells. B7-H6 is more highly expressed on tumors than on healthy tissue, and thus functions in antitumor immunosurveillance (7). Our data shows that rat NKp30 binds to human B7-H6.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Moretta, L. and Moretta, A. (2004) EMBO J. 23:255.
- Hsieh, C. et al. (2006) Eur. J. Immunol. 36:2170.
- Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
- Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
- Brandt, C. et al. (2009) J. Exp Med. 206:1495.
- Textor, S. et al. (2016) Oncoimmunology. 5:e1116674.
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