Recombinant Rat Nogo-A Fc Chimera Protein, CF Summary
(Thr544 - Phe725)
Accession # Q9JK11
(Pro100 - Lys330)
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rat Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved approximately 200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1, 2). Nogo-A in rat exists in four isoforms (3 - 5). The full length rat Nogo-A is 1163 aa in length and contains a 989 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa transmembrane connecting loop (SWISSPROT defines this region as being 94 aa in length while the original cloning papers identified it as being 66 aa in length). This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (6 - 8). Two other areas in the N-terminus have also been discovered to have bioactivity (6, 9, 10). The amino acid segment 59 - 172 is reported to block fibroblast spreading, while the aa segment 544 - 725 blocks neurite outgrowth and block fibroblast spreading (6, 10). The exact topology of Nogo-A is unclear. With two transmembrane segments, the N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (11, 12). Alternatively, the loop region and N-terminus may be on the same side of the membrane (6). Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (10, 13, 14). Rat Nogo-A is 78% aa identical to human Nogo-A overall, with 98% aa identical in the loop region and 81% aa identity in the aa 544 - 725 segment.
- Oertle, T. et al. (2003) FASEB J. 17:1238.
- GrandPre, T. et al. (2000) Nature 403:439.
- Chen, M.S. et al. (2000) Nature 403:434.
- Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
- Ito, T. and S.M. Schwartz (1999) GenBank Accession # Q9JK11.
- Oertle, T. et al. (2003) J. Neurosci. 23:5393.
- Fournier, A.E. et al. (2001) Nature 409:341.
- Wang, K.C. et al. (2002) Nature 420:74.
- Prinjha, R. et al. (2000) Nature 403:383.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
- Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
- Wang, X. et al. (2002) J. Neurosci. 22:5505.
- Acevedo, L. et al. (2004) Nat. Med. 10:382.
Citations for Recombinant Rat Nogo-A Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction
Authors: P Xiao, J Gu, W Xu, X Niu, J Zhang, J Li, Y Chen, Z Pei, J Zeng, S Xing
Sample Types: In Vivo
Applications: In Vivo
Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A.
Authors: Wright KT, El Masri W, Osman A, Roberts S, Chamberlain G, Ashton BA, Johnson WE
Biochem. Biophys. Res. Commun., 2007;354(2):559-66.
Sample Types: Whole Cells
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