Recombinant Rat Nogo-A Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2445-NG-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Recombinant Rat Nogo-A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 300 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived rat Nogo-A protein
Rat Nogo-A
(Thr544 - Phe725)
Accession # Q9JK11
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr544
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46.1 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions

Product Datasheets

You must select a language.

x

2445-NG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2445-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nogo-A

Rat Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved approximately 200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1, 2). Nogo-A in rat exists in four isoforms (3 - 5). The full length rat Nogo-A is 1163 aa in length and contains a 989 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa transmembrane connecting loop (SWISSPROT defines this region as being 94 aa in length while the original cloning papers identified it as being 66 aa in length). This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (6 - 8). Two other areas in the N-terminus have also been discovered to have bioactivity (6, 9, 10). The amino acid segment 59 - 172 is reported to block fibroblast spreading, while the aa segment 544 - 725 blocks neurite outgrowth and block fibroblast spreading (6, 10). The exact topology of Nogo-A is unclear. With two transmembrane segments, the N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (11, 12). Alternatively, the loop region and N-terminus may be on the same side of the membrane (6). Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (10, 13, 14). Rat Nogo-A is 78% aa identical to human Nogo-A overall, with 98% aa identical in the loop region and 81% aa identity in the aa 544 - 725 segment.

References
  1. Oertle, T. et al. (2003) FASEB J. 17:1238.
  2. GrandPre, T. et al. (2000) Nature 403:439.
  3. Chen, M.S. et al. (2000) Nature 403:434.
  4. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
  5. Ito, T. and S.M. Schwartz (1999) GenBank Accession # Q9JK11.
  6. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
  7. Fournier, A.E. et al. (2001) Nature 409:341.
  8. Wang, K.C. et al. (2002) Nature 420:74.
  9. Prinjha, R. et al. (2000) Nature 403:383.
  10. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  11. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
  12. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
  13. Wang, X. et al. (2002) J. Neurosci. 22:5505.
  14. Acevedo, L. et al. (2004) Nat. Med. 10:382.
Long Name
Reticulon 4A
Entrez Gene IDs
57142 (Human); 68585 (Mouse); 83765 (Rat)
Alternate Names
ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

Citations for Recombinant Rat Nogo-A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction
    Authors: P Xiao, J Gu, W Xu, X Niu, J Zhang, J Li, Y Chen, Z Pei, J Zeng, S Xing
    Autophagy, 2022-03-09;0(0):1-20.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A.
    Authors: Wright KT, El Masri W, Osman A, Roberts S, Chamberlain G, Ashton BA, Johnson WE
    Biochem. Biophys. Res. Commun., 2007-01-10;354(2):559-66.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Rat Nogo-A Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Rat Nogo-A Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Rat Nogo-A Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review