Recombinant Rat Reg3B Protein, CF

Catalog # Availability Size / Price Qty
8288-RG-050
Recombinant Rat Reg3B Protein, CF SDS-PAGE
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Recombinant Rat Reg3B Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.2-1.0 μg/mL.
Source
E. coli-derived rat Reg3B protein
Glu27-Gly175, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8288-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Rat Reg3B (Catalog # 8288-RG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 15.6 kDa and 14.5 kDa, respectively.

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Reconstitution Calculator

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Background: Reg3B

Rat Reg3B (Islets of Langerhans regenerating protein 3), also known as Reg-III alpha, Lithostathine 3, pancreatitis associated protein 2 and Lithostatine 3 is a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). Reg3B has been categorized in the type 3 subclass of the Reg gene family (3). The rat Reg3B cDNA encodes 174 amino acids (aa) including the 25 aa signal sequence and the 149 aa secreted mature protein. Rat Reg3B shares approximately 83% aa sequence identity with mouse Reg3B and 55% with humans Reg3B (1, 2). Reg proteins are thought to be stress response proteins that stimulate proliferation, particularly in  beta  cells of pancreatic islets (1). Reg3B is expressed primarily by acinar cells in the normal exocrine pancreas, and is up-regulated in NOD (non-obese diabetic) islets, hyperplastic islets, regenerating islets or in response to pancreatic injury or pancreatitis (1-6). IFN-beta causes Reg3B up-regulation in early-onset diabetic NOD mice or in a NOD insulinoma cell line (5). Reg3B is also up-regulated in mice lacking the pancreas-expressed keratin K8, and is thought to be regulated by keratin filament organization (4). It is proposed to serve as an autoantigen in NOD mouse immune-mediated type I diabetes models (5).

References
  1. Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
  2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
  3. Tebar LA. et al. (2008) PNAS. 105:11400.
  4. Zhong, B. et al. (2007) Mol. Biol. Cell 18:4969.
  5. Gurr, W. et al. (2007) Diabetes 56:34.
  6. Lu, Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 291:E50.
  7. Baeza, N. et al. (1997) FEBS Lett. 416:364.
  8. Planas, R. et al. (2006) Diabetologia 49:2379.
Long Name
Regenerating Islet-derived IIIb
Entrez Gene IDs
18489 (Mouse); 24618 (Rat)
Alternate Names
PAP1; Reg3B

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