Recombinant SARS-CoV-2 3CL Protease, CF Summary
Ser3264 - Gln3569
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||1.7 mg/ml (50 μM) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
GC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease (E-720) at a concentration of 54-71 nM.
SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease (Catalog # E-720) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.
LC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease (Catalog # E-720) was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.
Background: 3CL Protease
The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide. 3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid. Along with the CoV-2 Papain-Like Protease (catalog number E-611), 3CLpro presents an attractive target for therapeutic intervention for COVID-19. Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity. This recombinant protein is untagged.
- Chen Y.W., et al. (2020) F1000Research doi: 10.12688/f1000research.22457.2
- Pillaiyar T., et al. (2016) J. Med. Chem. 59: 6595
- Zhang L., et al. (2020) Science doi: 10.1126/science.abb3405
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