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Recombinant SARS-CoV-2 3CL Protease, CF

R&D Systems | Catalog # E-720

E. coli Expressed, Bioactivity Tested
R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant SARS-CoV-2 3CL Protease (E-720)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

YP_009725301.1

Applications

Bioactivity
View all 3CL Protease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived sars-cov-2 3CL Protease protein
Ser1 - Gln306

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

34 kDa

Activity

Recombinant SARS-CoV-2 3CL Protease is a cysteine protease that cleaves proteins with sequences including LQ(S/A/G).  The cleavage occurs c-terminal to the glutamine residue.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial SARS-CoV-2 3CL Protease concentration of 20-40 nM when using substrate S-720.

Scientific Data Images for Recombinant SARS-CoV-2 3CL Protease, CF

Recombinant SARS-CoV-2 3CL Protease Inhibition Activity

Recombinant SARS-CoV-2 3CL Protease Inhibition Activity

GC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease (E-720) at a concentration of 54-71 nM.

SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease.

SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease (Catalog # E-720) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.

LC-MS analysis of SARS-CoV-2 3CL Protease

LC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease (Catalog # E-720) was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.

Formulation, Preparation, and Storage

E-720
Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV-2 Papain-Like Protease (catalog number E-611), 3CLpro presents an attractive target for therapeutic intervention for COVID-19.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.  

References

  1. Chen Y.W., et al. (2020) F1000Res. 9:129.
  2. Pillaiyar T., et al. (2016) J. Med. Chem.  59: 6595.
  3. Zhang L., et al. (2020) Science 368:409.

Long Name

3CL Protease

Alternate Names

Main Protease, Mpro

UniProt

YP_009725301.1 (SARS-CoV-2)

Additional 3CL Protease Products

Product Documents for Recombinant SARS-CoV-2 3CL Protease, CF

Certificate of Analysis

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Product Specific Notices for Recombinant SARS-CoV-2 3CL Protease, CF

For research use only

Related Research Areas

Infectious Diseases

Citations for Recombinant SARS-CoV-2 3CL Protease, CF

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