Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein, CF

R&D Systems | Catalog # 10922-CV

Delta Plus Variant
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein (10922-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

YP_009724390.1

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein
SARS-CoV-2 AY.1 Spike
(Val16-Lys1211)(Thr19Arg, Thr95Ile, Gly142Asp, Glu156Gly, Phe157 del, Arg158 del, Trp258Leu, Lys417Asn, Leu452Arg, Thr478Lys, Asp614Gly, Pro681Arg, Asp950Asn) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro)
Accession # YP_009724390.1		 GCN4-IZ 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val16

Predicted Molecular Mass

138 kDa

SDS-PAGE

142-170 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).

Reviewed Applications

Read 1 review rated 5 using 10922-CV in the following applications:

Scientific Data Images for Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein, CF

Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His-tag Protein (Catalog # 10922-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 142-170 kDa.

Formulation, Preparation, and Storage

10922-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6,7). The S protein of SARS-CoV-2 shares 75% and 29% amino acid (aa) sequence identity with the S protein of SARS-CoV-1 and MERS, respectively. AY.1 is a sublineage of SARS-CoV-2 delta plus variant which carries the aa substitution L417N, L452R, and T478K  in the RBD. It was first identified in India and quickly spread to US through England and Japan. It is reported to partially escape neutralization of antibodies and vaccines (8).

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
  6. Li, W. et al. (2003) Nature 426:450.
  7. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  8. Kannan, S.R. et al. (2021) J. Autoimmun. 124:https://doi.org/10.1016/j.jaut.2021.102715.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_009724390.1

Additional Spike Products

Product Documents for Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein, CF

For research use only

Related Research Areas

Infectious Diseases

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    Recombinant SARS-CoV-2 AY.1 Spike (GCN4-IZ) His Protein, CF 10922-CV

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