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Recombinant Human ACE-2 Protein, CF

933-ZN 11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
933-ZN-010
ELISA data showing recombinant human ACE-2 binding to SARS-CoV-2 Spike RBD Protein
4 Images
Product Details
Citations (11)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ACE-2 Protein, CF Summary

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Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740, with a C-terminal 10-His tag
Accession #
Q9BYF1
N-terminal Sequence
Analysis
No results obtained: Gln18 predicted
Structure / Form
Recombinant Human ACE‑2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Predicted Molecular Mass
85 kDa
SDS-PAGE
101-111 kDa, reducing conditions

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

933-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Multiple Lots of Recombinant Human ACE-2 bind SARS-CoV-2 Spike RBD Protein View Larger

Four independent lots of recombinant Human ACE-2 His-tag 933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph to show lot-to-lot consistency of recombinant Human ACE-2.

Surface Plasmon Resonance (SPR) SPR Data of Recombinant Human ACE-2 binding to SARS-CoV-2 Spike RBD protein View Larger

Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of Kd=7.037 nM. (Biacore T200).

Enzyme Activity Enzymatic activity of Human ACE-2 View Larger

Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).

SDS-PAGE View Larger

1 μg/lane of Recombinant Human ACE-2 Protein (Catalog # 933-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at ~106 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12).  Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References
  1. Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
  2. Yan, et al. (2020) Science 367:1444.
  3. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Hoffmann, M. et al. (2020) Cell.181:1.
  6. Wrapp, et al. (2020) Science 367:1260.
  7. Imai, Y. et al. (2005) Nature 436:112.
  8. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  9. Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
  10. Jia, H. et al. (2016) Shock. 46:239.
  11. Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
  12. Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
  13. Crackower, M.A. et al. (2002) Nature 417:822.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citations for Recombinant Human ACE-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries
    J. Vet. Intern. Med., 2019;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  2. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries
    J. Vet. Intern. Med., 2019;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  3. Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm
    Authors: AM South, PA Nixon, MC Chappell, DI Diz, GB Russell, HA Shaltout, TM O'Shea, LK Washburn
    J. Pediatr., 2018;0(0):.
    Species: Human
    Sample Types: Urine
    Applications: Bioassay
  4. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-? and tau pathology
    Alzheimers Res Ther, 2016;8(1):50.
    Species: Human
    Sample Types: Fluorogenic Peptide Substrate
    Applications: Enzyme Assay
  5. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
    Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
    J Virol, 2014;88(2):1293-307.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms.
    Authors: Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, Pohlmann S, Simmons G
    J. Virol., 2011;84(17):8753-64.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  7. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
    Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
    J. Am. Coll. Cardiol., 2008;52(9):750-4.
    Species: N/A
    Sample Types: Fluorogenic Peptide Substrate
    Applications: Enzyme Assay
  8. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
    Authors: de Lang A, Osterhaus AD, Haagmans BL
    Virology, 2006;353(2):474-81.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry.
    Authors: Follis KE, York J, Nunberg JH
    Virology, 2006;350(2):358-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Angiotensin-converting enzyme 2 protects from severe acute lung failure.
    Authors: Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, Crackower MA, Fukamizu A, Hui CC, Hein L, Uhlig S, Slutsky AS, Jiang C, Penninger JM
    Nature, 2005;436(7047):112-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine.
    Authors: Tai W, He L, Zhang X, Pu J, Voronin D, Jiang S, Zhou Y, DU L
    Cell Mol Immunol, 0;17(6):613-620.
    Species: Human
    Sample Types: Whole Cells

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Fluorogenic Peptide Substrates

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

ES007
10 Citations
BA,BA

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