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Recombinant Human ACE-2 Protein, CF

Catalog #: 933-ZN
23 Citations 5 Reviews Datasheet / COA / SDS
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933-ZN-010
933-ZN-100
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Recombinant Human ACE‑2 Protein SEC-MALS.
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Product Details
Citations (23)
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Reviews (5)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ACE-2 Protein, CF Summary

Why choose R&D Systems ACE-2 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the Fc chimera ACE-2 protein here ACE-2 Fc Chimera

Other ACE-2 species available ACE-2 Proteins

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds with an ED50 of 1.00-12.0 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740, with a C-terminal 10-His tag
Accession #
Q9BYF1
N-terminal Sequence
Analysis
No results obtained: Gln18 predicted
Structure / Form
Recombinant Human ACE‑2 is prone to proteolytic cleavage at C-terminus. 
Predicted Molecular Mass
85 kDa
SDS-PAGE
101-111 kDa, reducing conditions

Product Datasheets

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933-ZN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

933-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

SEC-MALS View Larger

Recombinant Human ACE-2/His (Catalog # 933-ZN) has a molecular weight (MW) of 189.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Binding Activity ELISA data showing recombinant human ACE-2 binding to SARS-CoV-2 Spike RBD Protein View Larger

Four independent lots of recombinant Human ACE-2 His-tag 933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph to show lot-to-lot consistency of recombinant Human ACE-2. The ED50 for this effect is 1.00-12.0 ng/mL.

Surface Plasmon Resonance (SPR) SPR Sensorgram of Human ACE-2 binding to SARS-CoV-2 Spike RBD protein View Larger

Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of Kd=7.037 nM. (Biacore T200).

Enzyme Activity ELISA data showing recombinant human ACE-2 binding to SARS-CoV-2 Spike RBD Protein View Larger

Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).

SDS-PAGE View Larger

1 μg/lane of Recombinant Human ACE-2 Protein (Catalog # 933-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at ~106 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12).  Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References
  1. Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
  2. Yan, et al. (2020) Science 367:1444.
  3. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Hoffmann, M. et al. (2020) Cell.181:1.
  6. Wrapp, et al. (2020) Science 367:1260.
  7. Imai, Y. et al. (2005) Nature 436:112.
  8. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  9. Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
  10. Jia, H. et al. (2016) Shock. 46:239.
  11. Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
  12. Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
  13. Crackower, M.A. et al. (2002) Nature 417:822.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citations for Recombinant Human ACE-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Dual mechanism: Epigenetic inhibitor apabetalone reduces SARS-CoV-2 Delta and Omicron variant spike binding and attenuates SARS-CoV-2 RNA induced inflammation
    Authors: L Fu, D Gilham, SC Stotz, CD Sarsons, BD Rakai, LM Tsujikawa, S Wasiak, JO Johansson, M Sweeney, NCW Wong, E Kulikowski
    International immunopharmacology, 2023-02-23;117(0):109929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2
    Authors: X Guo, J Cao, JP Cai, J Wu, J Huang, P Asthana, SKK Wong, ZW Ye, S Gurung, Y Zhang, S Wang, Z Wang, X Ge, HY Kwan, A Lyu, KM Chan, N Wong, J Huang, Z Zhou, ZX Bian, S Yuan, HLX Wong
    Nature Communications, 2022-12-23;13(1):7907.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2
    Authors: JW Kim, SW Min, J Lee, HG Shin, HL Choi, HR Yang, JH Lee, YB Cho, H Shim, S Lee
    Biomedicines, 2022-12-17;10(12):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. The systemic renin-angiotensin system in COVID-19
    Authors: R Reindl-Sch, S Hödlmoser, O Domenig, K Krenn, F Eskandary, S Krenn, C Schörgenho, B Rumpf, M Karolyi, MT Traugott, A Abrahamowi, V Tinhof, H Mayfurth, V Rathkolb, S Mu beta nig, L Schmölz, R Ullrich, A Heinzel, F König, C Binder, D Bonderman, R Strassl, E Puchhammer, G Gorkiewicz, JH Aberle, B Jilma, C Wenisch, M Poglitsch, R Oberbauer, A Zoufaly, M Hecking
    Scientific Reports, 2022-11-22;12(1):20117.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  5. SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis
    Authors: B Bhargavan, GD Kanmogne
    International Journal of Molecular Sciences, 2022-09-09;23(18):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Molecular Interactions of Tannic Acid with Proteins Associated with SARS-CoV-2 Infectivity
    Authors: M Haddad, R Gaudreault, G Sasseville, PT Nguyen, H Wiebe, T Van De Ven, S Bourgault, N Mousseau, C Ramassamy
    International Journal of Molecular Sciences, 2022-02-27;23(5):.
    Applications: Bioassay
  7. ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury
    Authors: T Yamaguchi, M Hoshizaki, T Minato, S Nirasawa, MN Asaka, M Niiyama, M Imai, A Uda, JF Chan, S Takahashi, J An, A Saku, R Nukiwa, D Utsumi, M Kiso, A Yasuhara, VK Poon, CC Chan, Y Fujino, S Motoyama, S Nagata, JM Penninger, H Kamada, KY Yuen, W Kamitani, K Maeda, Y Kawaoka, Y Yasutomi, Y Imai, K Kuba
    Nature Communications, 2021-11-23;12(1):6791.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry
    Authors: SP Shukla, KB Cho, V Rustagi, X Gao, X Fu, SX Zhang, B Guo, DG Udugamasoo
    International Journal of Molecular Sciences, 2021-08-20;22(16):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  9. Metabolism of angiotensin peptides by angiotensin converting enzyme 2 (ACE2) and analysis of the effect of excess zinc on ACE2 enzymatic activity
    Authors: Y Polak, RC Speth
    Peptides, 2021-01-02;137(0):170477.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  10. Effect of angiotensin receptor blockers and angiotensin converting enzyme?2 on plasma equilibrium angiotensin peptide concentrations in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, T Huh, MA Oyama
    Journal of veterinary internal medicine, 2020-12-24;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  11. Effect of angiotensin receptor blockers and angiotensin-converting enzyme 2 on plasma equilibrium angiotensin peptide concentrations in cats with heart disease
    Authors: T Huh, É Larouche-L, KA Loughran, MA Oyama
    J Vet Intern Med, 2020-11-02;0(0):.
    Species: Feline
    Sample Types: Plasma
    Applications: Cell Culture
  12. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity.
    Authors: Lu J, Sun P
    J Biol Chem, 2020-10-29;295(52):18579-18588.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay, Surface Plasmon Resonance (SPR
  13. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity
    Authors: J Lu, PD Sun
    bioRxiv, 2020-07-01;0(0):.
    Applications: Enzyme Assay
  14. Physiological cyclic stretch up-regulates angiotensin-converting enzyme 2 expression to reduce proliferation and migration of vascular smooth muscle cells
    Authors: J Song, HY Qu, B Hu, C Bi, M Li, L Wang, XZ Huang, M Zhang
    Biosci. Rep., 2020-06-26;40(6):.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  15. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries
    J. Vet. Intern. Med., 2019-06-28;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  16. Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm
    Authors: AM South, PA Nixon, MC Chappell, DI Diz, GB Russell, HA Shaltout, TM O'Shea, LK Washburn
    J. Pediatr., 2018-11-05;0(0):.
    Species: Human
    Sample Types: Urine
    Applications: Bioassay
  17. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-? and tau pathology
    Alzheimers Res Ther, 2016-11-25;8(1):50.
    Species: Human
    Sample Types: Fluorogenic Peptide Substrate
    Applications: Enzyme Assay
  18. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
    Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
    J Virol, 2013-11-13;88(2):1293-307.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  19. A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms.
    Authors: Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, Pohlmann S, Simmons G
    J. Virol., 2010-06-23;84(17):8753-64.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  20. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
    Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
    J. Am. Coll. Cardiol., 2008-08-26;52(9):750-4.
    Applications: Enzyme Assay
  21. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
    Authors: de Lang A, Osterhaus AD, Haagmans BL
    Virology, 2006-07-24;353(2):474-81.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry.
    Authors: Follis KE, York J, Nunberg JH
    Virology, 2006-03-07;350(2):358-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Angiotensin-converting enzyme 2 protects from severe acute lung failure.
    Authors: Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, Crackower MA, Fukamizu A, Hui CC, Hein L, Uhlig S, Slutsky AS, Jiang C, Penninger JM
    Nature, 2005-07-07;436(7047):112-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Fluorogenic Peptide Substrates

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

ES007
18 Citations
2 Reviews
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Reviews for Recombinant Human ACE-2 Protein, CF

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Recombinant Human ACE-2 Protein, CF
By Anonymous on 08/24/2021
Application: Enzymatic activity in vitro
Reason for Rating: Very consistent results for enzymatic assay using either the fluorescent substrate Mca-APK(Dnp) or Angiotensin II.
Enzymatic activity in vitro Recombinant Human ACE-2 Protein, CF 933-ZN
Recombinant Human ACE-2 Protein, CF
By Anonymous on 11/21/2020
Application: In vitro bioactivity in cell culture
Recombinant Human ACE-2 Protein, CF
By Anonymous on 10/23/2020
Application: Enzymatic activity in vitro
Recombinant Human ACE-2 Protein, CF
By Riddhi Joshi on 08/05/2016
Recombinant Human ACE-2 Protein, CF 933-ZN
Recombinant Human ACE-2 Protein, CF
By Shrinidh Joshi on 08/05/2016
Recombinant Human ACE-2 Protein, CF 933-ZN