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Recombinant Human ACE-2 Protein, CF

933-ZN 10 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
933-ZN-010
Recombinant Human ACE-2 Protein, CF Bioactivity
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ACE-2 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740, with a C-terminal 10-His tag
Accession #
Q9BYF1
N-terminal Sequence
Analysis
No results obtained: Gln18 predicted
Structure / Form
Recombinant Human ACE‑2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Predicted Molecular Mass
85 kDa
SDS-PAGE
101-111 kDa, reducing conditions

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

933-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Bioactivity View Larger

Recombinant Human ACE-2 His-tag (933-ZN) binds Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (10499-CV) in a functional ELISA.

Enzyme Activity View Larger

Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

References
  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 102130864 (Cynomolgus Monkey)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citations for Recombinant Human ACE-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries
    J. Vet. Intern. Med., 2019;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  2. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
    Authors: É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries
    J. Vet. Intern. Med., 2019;0(0):.
    Species: Canine
    Sample Types: Plasma
    Applications: Bioassay
  3. Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm
    Authors: AM South, PA Nixon, MC Chappell, DI Diz, GB Russell, HA Shaltout, TM O'Shea, LK Washburn
    J. Pediatr., 2018;0(0):.
    Species: Human
    Sample Types: Urine
    Applications: Bioassay
  4. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-? and tau pathology
    Alzheimers Res Ther, 2016;8(1):50.
    Species: Human
    Sample Types: Fluorogenic Peptide Substrate
    Applications: Enzyme Assay
  5. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
    Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
    J Virol, 2014;88(2):1293-307.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms.
    Authors: Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, Pohlmann S, Simmons G
    J. Virol., 2011;84(17):8753-64.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  7. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
    Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
    J. Am. Coll. Cardiol., 2008;52(9):750-4.
    Species: N/A
    Sample Types: Fluorogenic Peptide Substrate
    Applications: Enzyme Assay
  8. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
    Authors: de Lang A, Osterhaus AD, Haagmans BL
    Virology, 2006;353(2):474-81.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry.
    Authors: Follis KE, York J, Nunberg JH
    Virology, 2006;350(2):358-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Angiotensin-converting enzyme 2 protects from severe acute lung failure.
    Authors: Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, Crackower MA, Fukamizu A, Hui CC, Hein L, Uhlig S, Slutsky AS, Jiang C, Penninger JM
    Nature, 2005;436(7047):112-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

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Fluorogenic Peptide Substrates

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

ES007
10 Citations
1 Review
BA,BA

Reviews for Recombinant Human ACE-2 Protein, CF

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Recombinant Human ACE-2 Protein, CF
By Anonymous on 08/05/2016
Application:
Serum Recombinant Human ACE-2 Protein, CF 933-ZN
Recombinant Human ACE-2 Protein, CF
By Anonymous on 08/05/2016
Application:
Serum Recombinant Human ACE-2 Protein, CF 933-ZN