Recombinant SARS-CoV-2 B.1.2 (+D399N) N (1-419) Protein, CF Summary
Met1-Ala419 (Pro67Ser, Pro199Leu, Asp399Asn), with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human SARS-CoV-2 B.1.2 (+D399N) N (1-419) Protein (Catalog # 11216-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.
SARS-Cov-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-Cov-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains a RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain (2,3). The SARS-Cov-2 N protein shares 91% and 47% amino acid sequence identity with SARS-Cov-1 and MERS N protein, respectively. The SARS-Cov-2 N protein displays VSR (viral suppressor of RNA interference) activity in mammalian cells (4). As the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (5, 6), it has been used to develop diagnostic kit for detecting IgM and IgG antibodies against SARS-Cov-2 (7). Several emerging SARS-CoV-2 genomes have been identified with mutations compared to the Wuhan-Hu-1 SARS-CoV-2 reference sequence, including the B.1.2 variant. Within the N protein, there are 2 mutations, P67S and P199L, in the B.1.2 variant which might make attractive targets for the development of antiviral therapeutics or potential diagnostic tools. Additionally, the D399N mutation has been reported to affect the performance of commercial antigen tests (8).
- Wu, F. et al. (2020) Nature 579:265.
- Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59.
- Hurst, K.R. et al. (2009) J. Virol. 83:7221.
- Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1.
- Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
- Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
- Liu, W. et al. (2020) J. Clin. M icrobiol. doi: 10.1128/JCM.00461-20.
- Bourassa L. et al. (2021) J. Clin. Virol. 141:104900.
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