Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His Protein, CF

Catalog # Availability Size / Price Qty
11260-CV-100
Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His-tag Protein Binding Activity.
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Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 Fc Chimera (Catalog # 10544-ZN).
Source
Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike RBD protein
Human SARS-CoV-2 BA.2.75 Spike RBD
(Val16-Lys1211)
(Thr19Ile, Leu24del, Pro25del, Pro26del, Ala27Ser, Gly142Asp, Lys147Glu, Trp152Arg, Phe157Leu, Ile210Val, Val213Gly, Gly257Ser, Gly339His, Ser371Phe, Ser373Pro, Ser375Phe, Thr376Ala, Asp405Asn, Arg408Ser, Lys417Asn, Asn440Lys, Gly446Ser, Asn460Lys, Ser477Asn, Thr478Lys, Glu484Ala, Gln498Arg, Asn501Tyr, Tyr505His, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Arg682Ser, Arg685Ser, Asn764Lys, Asp796Tyr, Gln954His, Asn969Lys, Lys986Pro, Val987Pro)
Accession # YP_009724390.1
GCN4-IZ6-His tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val16
Predicted Molecular Mass
138 kDa
SDS-PAGE
143-170 kDa, under reducing conditions.

Product Datasheets

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11260-CV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11260-CV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His-tag Protein Binding Activity. View Larger

Recombinant SARS-CoV-2 BA.2.75 Spike RBD (GCN4-IZ) His-tag (Catalog # 11260-CV) binds Recombinant Human ACE-2 Fc Chimera (10544-ZN) in a functional ELISA.

SDS-PAGE Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His-tag Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant SARS-CoV-2 BA.2.75 S (GCN4-IZ) His-tag Protein (Catalog # 11260-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 143-170 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background:

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS-CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5).  The S Protein of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds a metallopeptidase, Angiotensin-Converting Enzyme 2 (ACE-2), but with much higher affinity and faster binding kinetics through the receptor binding domain (RBD) located in the C-terminal region of S1 subunit (6). It has been demonstrated that the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (7, 8). Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (9). There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS-CoV-2 (10).  The S protein of SARS-CoV-2 shares 75% and 29% aa identity with S protein of SARS-CoV-1 and MERS, respectively. Several emerging SARS-CoV-2 genomes have been identified including the Omicron, or B.1.1.529, variant. First identified in November 2021 in South Africa, the Omicron variant quickly became the predominant SARS-CoV-2 variant and is considered a variant of concern (VOC). The Omicron variant contains 32 mutations in the S protein, 3 to 4 times more than in other SARS-CoV-2 variants, that potentially affect viral fitness and transmissibility (11). Of these mutations,15 are located in the RBD domain and allow the Omicron variant to bind ACE-2 with greater affinity and, potentially, increased transmissibility (11, 12). Several additional mutations throughout the S protein have been shown or are predicted to enhance spike cleavage and could aid transmission (13-15). The study of the Omicron variant's impact on immune escape and reduced neutralization activity to monoclonal antibodies along with an increased risk of reinfection, even among vaccinated individuals, remains ongoing (16). TThe BA.2.75 subvariant was reported to show significantly higher binding affinity to ACE-2 compared to another dominant subvariant BA.5 (17).

References
  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
  6. Ortega, J.T. et al. (2020) EXCLI J. 19:410.
  7. Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1.
  8. Isabel, S. et al. (2020) Sci Rep. 10, 14031. https://doi.org/10.1038/s41598-020-70827-z.
  9. Tai, W. et al. (2020) Cell. Mol. Immunol. 17:613.
  10. Okba, N. M. A. et al. (2020). Emerg. Infect. Dis. https://doi.org/10.3201/eid2607.200841.
  11. Shah, M. and Woo, H.G. (2021) bioRxiv https://doi.org/10.1101/2021.12.04.471200.
  12. Lupala, C.S. et al. (2021) bioRxiv https://doi.org/10.1101/2021.12.10.472102.
  13. Zhang, L. et al. (2020) Nat Commun. 11:6013.
  14. Lasek-Nesselquist, E. et al. (2021) medRxiv https://doi.org/10.1101/2021.03.10.21253285.
  15. Scheepers, C. et al. (2021) medRxiv https://doi.org/10.1101/2021.08.20.21262342.
  16. Callaway, E. and Ledford, H. (2021) Nature 600:197.
  17. Cao, Y. et al. (2022) bioRxiv  https://doi.org/10.1101/2022.07.18.500332.

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