Recombinant SARS-CoV-2 NSP9 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10631-CV-100
Recombinant SARS-CoV-2 NSP9 His-tag Protein SDS-PAGE.
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Recombinant SARS-CoV-2 NSP9 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Source
E. coli-derived sars-cov-2 NSP9 protein
Asn1-Gln113
with N-terminal Met and C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
13 kDa
SDS-PAGE
13 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10631-CV

Formulation Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE Recombinant SARS-CoV-2 NSP9 His-tag Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant SARS-CoV-2 NSP9 His-tag (Catalog # 10631-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 13 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

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Background: NSP9

Non-structural protein 9 (NSP9) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP9 is a small 113 amino acid protein that forms a biologically active homodimer where each monomer consists of a beta barrel and C-terminal helical domain motif that promotes obligate dimerization (2,3). NSP9 is capable of binding nucleic acids in a nonsequence-specific manner with a preference of a single stranded RNA (4,5) although disruption of the dimeric interface appears to impact RNA binding (6). The NSP9 sequence is conserved across coronaviruses (3). NSP9 was shown to interact with other viral NSP proteins including NSP7, NSP8, and NSP12 (5,7,8). In addition, NSP9 has been shown to bind host cell proteins including DEAD-box RNA helicase 5 (DDX5), the ubiquitin E3 ubiquitin ligase MIB1, and elongation factor eIF4H in SARS-CoV2 and related viruses (9,10). The interactions of NSP9 with these host cell proteins promote viral replication (9,10) supporting the conclusion that NSP9 is important for virulence (2,3).

References
  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Miknis, Z.J. et al. (2009) J. Virol. 83:3007. 
  3. Littler, D.R. et al. (2020) iScience 23:101258
  4. Egloff, M.P. et al. (2004) Proc. Nat. Acad. Sci. U.S.A. 101:3792.
  5. Sutton, G. et. al. (2004) Structure 12:341.
  6. Hu, T. et al. (2017) Protein Sci. 26:1037.
  7. von Brunn, A. et al. (2007) PLoS One 2:e459.
  8. Chen, J. et al. (2017) Front. Microbiol. 8:853.
  9. Zhao, S. et al. (2015) Virus Res. 195:217.
  10. Gordon, D.E. et al. (2020) Nature 583:459.
Long Name
Non-Structural Protein 9
Alternate Names
Non-structural protein 9; NSP9; ORF1a polyprotein; ORF1ab polyprotein; p12

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