Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

R&D Systems | Catalog # E-611

E. coli Expressed, Bioactivity Tested
R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant SARS-CoV-2 GST-Papain-like Protease (E-611)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

YP_009725299.1

Applications

Bioactivity
View all Papain-like Protease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived sars-cov-2 Papain-like Protease protein
Glu746 - Lys1060, with an N-terminal GST-tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

62 kDa

Activity

Recombinant SARS-CoV-2 virus Papain-like protease (PLPro) is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rhodamine 110 (U-555) substrate.  When using polyubiquitin chain substrate(s), this enzyme demonstrates a preference for K48 linkages.

Scientific Data Images for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

Recombinant SARS-CoV-2 GST-Papain-like Protease SDS-PAGE

Recombinant SARS-CoV-2 GST-Papain-like Protease SDS-PAGE

0.5 μg and 1 μg of Recombinant SARS-CoV-2 GST-Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Formulation, Preparation, and Storage

E-611
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Papain-like Protease

The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity.  In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways.  When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.  

References

  1. Clasman J.R., et al. (2020) Antiviral Res.  174: 104661.
  2. Frieman M., et al. (2009) J. Virol.  83: 6689.
  3. Lindner H.A., et al. (2007) Arch. Biochem. Biophys.  466: 8.
  4. Ratia K., et al. (2014) PLoS Pathog.  doi: 10.1371/journal.ppat.1004113.

Alternate Names

PL-PRO, PLpro, pp1a, Replicase polyprotein 1a

UniProt

YP_009725299.1 (SARS-CoV-2)

Additional Papain-like Protease Products

Product Documents for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

For research use only

Citations for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

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FAQs for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    A: Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catalog #E-611 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

  • Q: Is there a molar extinction coefficient for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    A: We have not determined the molar extinction coefficient of E-611 as this is not a regular QC specification. However, a theoretical E280 extinction coefficient can be produced using Expasy software based on the amino acid sequence, which includes the GST tag in the N-terminus.

  • Q: Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    A: Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catalog #E-611 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

  • Q: Is there a molar extinction coefficient for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    A: We have not determined the molar extinction coefficient of E-611 as this is not a regular QC specification. However, a theoretical E280 extinction coefficient can be produced using Expasy software based on the amino acid sequence, which includes the GST tag in the N-terminus.

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