Recombinant SARS-CoV-2 Papain-like Protease, CF

Catalog # Availability Size / Price Qty
E-611-050
Recombinant SARS-CoV-2 Papain-like Protease, CF SDS-PAGE
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Recombinant SARS-CoV-2 Papain-like Protease, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant SARS-CoV-2 virus Papain-like protease (PLPro) is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rhodamine 110 (U-555) substrate.  When using polyubiquitin chain substrate(s), this enzyme demonstrates a preference for K48 linkages.
Source
E. coli-derived sars-cov-2 Papain-like Protease protein
Glu746 - Lys1060
Accession #
Predicted Molecular Mass
62 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-611

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE View Larger

0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

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Reconstitution Calculator

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Background: Papain-like Protease

The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 or Wuhan 2019 coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity.  In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways.  When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.  This protein contains an N-terminal GST tag.

References
  1. Clasman J.R., et al. (2020) Antiviral Res.  174: 104661
  2. Frieman M., et al. (2009) J. Virol.  83: 6689
  3. Lindner H.A., et al. (2007) Arch. Biochem. Biophys.  466: 8
  4. Ratia K., et al. (2014) PLoS Pathog.  doi: 10.1371/journal.ppat.1004113
Alternate Names
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a

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