Recombinant Viral 136R/Y136 Protein

Type I and III Interferon Inhibitor.
  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Type-I and Type-III IFN-mediated anti-viral activity. Huang, J. et al. (2007) PANS 104:9822. The ED50 for this effect is 2-12 ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta (Catalog # 8499-IF).
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Viral 136R/Y136
    (Tyr17-Lys351)
    Accession # Q9DHH7
    HPGGGSGGGSGGGS HHHHHH
    N-terminusC-terminus
  • N-terminal Sequence
    Analysis
    Tyr17
  • Predicted Molecular Mass
    41 kDa
  • SDS-PAGE
    88-120 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8976-BR
 
8976-BR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 136R
136R, also known as Y136, is an approximately 80 kDa glycosylated and secreted protein encoded by the Yaba-like disease virus genome (1). 136R binds directly to type I and type III interferons (IFN-alpha and IFN-lambda) (1). It prevents the interaction of rhesus and human IFN-alpha as well as human IFN-beta with the IFN-gamma R1 complex, although it shares only 14% amino acid sequence identity with the ECD of human IFN-gamma R1 (1). 136R blocks STAT1 signaling and the anti-viral protective functions of IFN-alpha, -beta, -omega, -kappa, and -lambda 1/IL-29 (1).
  • References:
    1. Huang, J. et al. (2007) Proc. Natl. Acad. Sci. USA 104:9822.
  • Long Name:
    Soluble Type I Interferon Receptor 136R
  • Alternate Names:
    136R
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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