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Recombinant Human IFN-beta Protein

R&D Systems | Catalog # 8499-IF

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human IFN-beta Protein (8499-IF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P01574

Applications

Bioactivity
View all IFN-beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22 & Ser23

Predicted Molecular Mass

20 kDa

SDS-PAGE

20-26 kDa, reducing conditions

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 5.00-30.0 pg/mL.

Reviewed Applications

Read 2 reviews rated 3.5 using 8499-IF in the following applications:

Scientific Data Images for Recombinant Human IFN-beta Protein

Recombinant Human IFN-beta Protein Binding Activity

Recombinant Human IFN-beta Protein Binding Activity

Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5.00-30.0 pg/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8499-IF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200-500 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8499-IF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

References

  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt

P01574

Additional IFN-beta Products

Product Documents for Recombinant Human IFN-beta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8499-IF (with carrier)
Download SDS 8499-IF/CF (carrier free)

Product Specific Notices for Recombinant Human IFN-beta Protein

For research use only

Citations for Recombinant Human IFN-beta Protein

Customer Reviews for Recombinant Human IFN-beta Protein (2)

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FAQs for Recombinant Human IFN-beta Protein

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  • Q: Is there a mass to unit conversion for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF)?

    A: We have developed an approximate mass to unit conversion factor for Recombinant Human IFN-beta Protein (Catalog # 8499-IF & 8499-IF/CF) based on testing against WHO International Standard (NIBSC code: 00/572). In this testing, we found a relative conversion of 1 ug of cytokine = 2.8 x 105 Units. This information can be found in our Unit Conversion Table: https://www.rndsystems.com/resources/technical-information/unit-conversion-table Please note this information is for reference purposes only and is not routinely tested.

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