Recombinant Viral B18R Protein, CF

Type I IFN Inhibitor
Get 50% off R&D Systems Recombinant Viral B18R Protein! Use Promo Code: B18R50
View Terms and Conditions
    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. Symons, J.A. et al. (1995) Cell 81:551. The ED50 for this effect, as measured by inhibition of Recombinant Human IFN‑ alpha 2 (Catalog # 11105-1) , is 0.3-1.8 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived viral B18R protein
    MD Human IgG1
    (Pro100-Lys300)
    IEGR Viral B18R
    (His20-Glu351)
    Accession # P25213
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Met-Asp-Pro100
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    65 kDa (monomer)
  • SDS-PAGE
    80-95 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

8185-BR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
The viral type I IFN receptor, B18R, inhibits the anti-viral activity of Recombinant Human IFN-alpha 2. The ED50 for this effect is 0.3-1.8 ng/mL.
Background: B18R
B18R (Soluble interferon alpha/beta receptor B18) is a 60-65 kDa protein encoded by the Vaccinia virus genome and by the genomes of other orthopoxviruses. Its function represents one of several mechanisms used by these viruses to evade the host immune response (1, 2). It is known as B18R in the Western Reserve (WR) strain of Vaccinia but as B19R in the Copenhagen strain (3). There is a structurally-unrelated, larger Vaccinia protein that is also known as B18R (or B16R) that contains multiple ankyrin-like repeats (4). The soluble interferon receptor B18R, however, contains three immunoglobulin-like domains and shows homology to human, mouse, and bovine type I interferon receptors (5). The Wyeth strain of Vaccinia virus encodes a truncated protein that lacks the C-terminal Ig-like domain, and B18R is functionally absent in the Lister strain (6, 7). B18R functions as a decoy receptor for type I interferons (IFN alpha, beta, omega). It binds to type I interferons from multiple species and prevents IFN signaling through its receptors (6-8). B18R binds to the surface of virus infected and uninfected cells where it retains its capacity to bind and neutralize IFN (6, 8). It shields those cells from the antiviral effects of type I interferons, thereby enabling virus replication and pathogenicity (6-8). B18R also limits the effectiveness of IFN alpha produced following TLR activation (9), and it limits adaptive T cell responses (3). B18R is a common factor used for increasing the efficiency of RNA reprogramming in induced pluripotent stem cells (IPS cells) (10).

  • References:
    1. Smith, G.L. et al. (2013) J. Gen. Virol. 94:2367.
    2. Perdiguero, B. and M. Esteban (2009) J. Interferon Cytokine Res. 29:581.
    3. Gomez, C.E. et al. (2012) J. Virol. 86:5026.
    4. Goebel, S.J. et al. (1990) Virology 179:247.
    5. Smith, G.L. and Y.S. Chan (1991) J. Gen. Virol. 72:511.
    6. Alcami, A. et al. (2000) J. Virol. 74:11230.
    7. Symons, J.A. et al. (1995) Cell 81:551.
    8. Colamonici, O.R. et al. (1995) J. Biol. Chem. 270:15974.
    9. Waibler, Z. et al. (2009) J. Virol. 83:1563.
    10. Warren, L. et al. (2010) Cell Stem Cell. 7:618.
  • Long Name:
    Soluble Interferon alpha/beta Receptor B18
  • Entrez Gene IDs:
    3707577 (Viral)
  • Alternate Names:
    B18R; B19R
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Viral B18R Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
You May Also Like
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee