Recombinant Viral B8R Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit rhIFN-gamma mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. Th ED50 for this effect is 0.5-3 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Lys18-Ser272, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Lys18
  • Structure / Form
    Noncovalently-linked homodimer
  • Predicted Molecular Mass
    30 kDa (monomer)
  • SDS-PAGE
    37-43 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8225-BR/CF
 
8225-BR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
B8R Inhibits IFN-gamma Activity.
The viral receptor B8R inhibits IFN-gamma-mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. The ED50 for this effect is 0.5-3 ng/mL.
1 μg/laneof Recombinant Viral B8R (Catalog # 8225-BR/CF) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at
37-43 kDa and NR bands at 67-92 kDa.
Background: B8R
B8R is a secreted Interferon gamma (IFN-gamma ) binding protein encoded by the open reading frame of the Vaccina virus (smallpox) (1, 2). B8R is a 43 kDa protein and shares only 19% amino acid sequence identity with the extracellular region of the human IFN-gamma Receptor 1 (IFN-gamma R1). However, B8R binds IFN-gamma orthologs from multiple species including human, rat, rabbit, bovine, and chicken with high affinity, but it binds mouse IFN-gamma with low affinity (1, 3, 4). During infection, virally-induced B8R binds and sequesters endogenous IFN-gamma, thereby suppressing the host immune response and promoting viral immune evasion (5). While B8R is not required for viral replication, it contributes significantly to Vaccinia virus virulence and is frequently inactivated prior to Vaccinia use in vaccines (5-9). Peptide IFN-gamma mimetics that activate IFN-gamma R1 and are not bound and sequestered by B8R have been developed for research and therapeutic use (10, 11).
  • References:
    1. Alcami, A. and G.L. Smith (1995) J. Virol. 69:4633.
    2. Goebel, S.J. et al. (1990) Virology 179:247.
    3. Puehler, F. et al. (1998) Virology 248:231.
    4. Mossman, K. et al. (1995) Virology 208:762.
    5. Smith, G.L. et al. (2013) J. Gen. Virol. 94:2367.
    6. Symons, J.A. et al. (2002) J. Gen. Virol. 83:1953.
    7. Verardi, P.H. et al. (2001) J. Virol. 75:11.
    8. Denes, B. et al. (2006) J. Gene Med. 8:814.
    9. Dimier, J. et al. (2011) J. Virol. 85:5016.
    10. Ahmed, C.M. et al. (2007) J. Immunol. 178:4576.
    11. Ahmed, C.M. et al. (2005) J. Virol. 79:5632.
    12.  
  • Long Name:
    Soluble Interferon alpha/beta Receptor B8
  • Entrez Gene IDs:
    3707661 (Viral)
  • Alternate Names:
    B8R
Related Research Areas
Primary Antibodies
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His Tag PE-conjugated Antibody

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His Tag Antibody

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His Tag Alexa Fluor® 488-conjugated Antibody

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His Tag PerCP-conjugated Antibody

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His Tag Alexa Fluor® 700-conjugated Antibody

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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

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