Recombinant Viral CKBP Protein

R&D Systems | Catalog # 8254-CK

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Viral CKBP Protein (8254-CK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P19063

Applications

Bioactivity
View all CKBP Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral CKBP protein
Pro18-Val258, with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

27 kDa

SDS-PAGE

36-40 kDa, reducing conditions

Activity

Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5.
The ED50 for this effect is 0.02-0.1 μg/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8254-CK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8254-CK/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CKBP

Chemokine-binding Protein (CKBP), also known as, CKBP-II, vCCI, or B29R, is a 35 kDa secreted protein encoded by multiple poxviruses, including the Vaccinia virus (Lister strain) (1). While CKBP binds and sequesters CC chemokines with high affinity, it shares little amino acid sequence identity with CC chemokine receptors (1-3). CKBP prevents CC chemokine activity by masking the chemokine/chemokine receptor binding site (4). Chemokine-binding proteins encoded by poxvirus and herpesvirus strains inhibit the recruitment of immune cells and contribute to viral evasion of host immune responses (5). Local administration of pan-CC chemokine binding proteins such as CKBP have been shown to reduce inflammation resulting from allergic airway hyperactivity and arthritis (6, 7).

References

  1. Alcami, A. et al. (1998) J. Immunol. 160:624.
  2. Kuo, N.W. et al. (2014) J. Biol. Chem. [In press].
  3. Zhang, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:13985.
  4. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
  5. Seet, B.T. and G. McFadden (2002) J. Leukoc. Biol. 72:24.
  6. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
  7. Buatois, V. et al. (2010) J. Immunol. 185:2544.

Long Name

Chemokine-binding Protein

Alternate Names

B29R, C23L

UniProt

P19063 (Viral)

Additional CKBP Products

Product Documents for Recombinant Viral CKBP Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8254-CK (with carrier)
Download SDS 8254-CK/CF (carrier free)

Product Specific Notices for Recombinant Viral CKBP Protein

For research use only

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