Recombinant Viral CMV UL146/vCXC1 Protein

    
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  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.3-0.6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.03-0.18 µg/mL.
  • Source
    E. coli-derived viral UL146/vCXC1 protein
    Thr23-Gly117
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr23
  • Predicted Molecular Mass
    11.1 kDa
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
620-CM
 
620-CM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UL146/vCXC1

Cytomegalovirus (CMV), a member of the beta herpesvirus subfamily, typically causes subclinical or latent infections in the normal adult population. However, CMV can cause congenital disease during pregnancy and is a human opportunistic pathogen that affects immunocompromised individuals. The CMV genome has been shown to contain homologs of cellular immunomodulatory proteins, including US28 (a CC chemokine receptor) and a MHC class I homolog. Virulent CMV clinical isolates have also been shown to carry at least 19 genes, designated UL133 - UL151, that are not found in laboratory strains that have lost virulence characteristics. Two of these genes, UL146 and UL147, exhibit sequence similarity to CXC chemokines.

The CMV UL146 open-reading frame encodes a 117 amino acid residue precursor protein with a predicted 22 residues signal peptide that is cleaved to generate the mature protein. Recombinant UL146 has been shown to induce calcium mobilization, chemotaxis and degranulation of neutrophils.

  • References:
    1. Dairaghi, D. et al. (1998) Sem. Virol. 8:377.
    2. Cha, T.A. et al. (1996) J. Virol.70:78.
  • Long Name:
    Cytomegalovirus UL146
  • Alternate Names:
    HHV5wtgp117; UL146; vCXC1; vCXCL1; Y18
Related Research Areas

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