Recombinant Viral MIP-I Protein

Carrier Free

Catalog # Availability Size / Price Qty
600-VA-025/CF

With Carrier

Catalog # Availability Size / Price Qty
600-VA-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
FAQs
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Recombinant Viral MIP-I Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 2-6 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8. The ED50 for this effect is 4-16 ng/mL.
Source
E. coli-derived viral MIP-I protein
Ala25-Ala95
Accession #
N-terminal Sequence
Analysis
Ala25
Predicted Molecular Mass
7.9 kDa

Product Datasheets

600-VA (with carrier)

600-VA/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

600-VA

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

600-VA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIP-I

Human herpesvirus-8 (HHV-8)/Kaposi’s sarcoma-associated herpesvirus (KSHV) is a gamma  herpesvirus with homology to herpesvirus Saimiri and Epstein-Barr virus. HHV-8 is etiologically linked to Kaposi’s sarcoma and a B-cell lymphoma known as primary effusion lymphoma. HHV-8 has been shown to encode a variety of immunomodulatory proteins which were apparently pirated from cellular genes by the virus. Three chemokine-like proteins, vMIP-I, vMIP-II and vMIP-III have been found to be encoded within the HHV-8 genome.

Viral MIP-I (also termed vMIP-1 alpha ) cDNA encodes a 95 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide that is cleaved to yield a 71 aa residue mature protein. Among human chemokines, vMIP-I is most closely related to MIP-1 alpha, sharing approximately 38% amino acid sequence identity. At the amino acid sequence level, vMIP-I and vMIP-II also share 48% identity. vMIP-I and vMIP-II are more closely related to one another phylogenetically than to other human chemokines, suggesting that they may have arisen by gene duplication within the virus rather than by two independent gene aquisitions. Both vMIP-I and vMIP-II have been shown to partially block HIV infection of peripheral blood mononuclear cells. vMIP-I and vMIP-II have also been found to be highly angiogenic in the chorioallantoic assay, suggesting that they may be partially responsible for the marked vascularity seen in KSHV-associated tumors.

References
  1. Moore, P.S. et al. (1996) Science 274:5293.
  2. Boshoff, C. et al. (1997) Science 278:290.
Long Name
Macrophage Inflammatory Protein I
Alternate Names
MIPI; MIP-I

Citations for Recombinant Viral MIP-I Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Genetic fusions with viral chemokines target delivery of nonimmunogenic antigen to trigger antitumor immunity independent of chemotaxis.
    Authors: Ruffini PA, Biragyn A, Coscia M, Harvey LK, Cha SC, Bogen B, Kwak LW
    J. Leukoc. Biol., 2004;76(1):77-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Yaba-like disease virus protein 7L is a cell-surface receptor for chemokine CCL1.
    Authors: Najarro P, Lee HJ, Fox J, Pease J, Smith GL
    J. Gen. Virol., 2003;84(0):3325-36.
    Species: Virus
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
    Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
    Eur. J. Pharmacol., 2002;456(1):1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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