Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF Summary
Recombinant Yeast GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.
Met1 - Leu1024 with a N-terminal GST (glutathione S-transferase) tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Ubiquitin-activating Enzyme/UBE1
Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1024 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 114 kDa. Human and mouse UBE1 share 52% and 53% aa sequence identity with the yeast UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys600 in S. cerevisiae, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating-Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11). UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.
- Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
- Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
- Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Schulman, B.A. and J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
- Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
- Haas, A.L. and I.A. Rose (1982) J. Biol. Chem. 257:10329.
- Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
- Nouspikel, T. and P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
- Moundry, P. et al. (2012) Cell Cycle 11:1573.
- Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Citations for Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 affect ubiquitination activity and decrease enzyme stability
Authors: PK Shukla, D Sinha, AM Leng, JE Bissell, S Thatipamul, R Ganguly, KS Radmall, JJ Skalicky, DC Shrieve, MB Chandrasek
The Journal of Biological Chemistry, 2022;0(0):102524.
Sample Types: Recombinant Protein
Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin
Authors: S Lee, JM Tumolo, AC Ehlinger, KK Jernigan, SJ Qualls-His, PC Hsu, WH McDonald, WJ Chazin, JA MacGurn
Sample Types: Protein
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