Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16 - 18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet β-cells and intestinal villi. It is upregulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65% - 68% aa sequence identity with mouse and rat Reg1A.
Rat Reg1A ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-82146
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Key Product Details
Sample Type & Volume Required Per Well
Serum, Plasma, Cell supernatant (100 uL)
Sensitivity
18.75 pg/mL
Assay Range
31.25 - 2000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat Reg1A in samples. No Significant cross-reactivity or interference between Rat Reg1A and analogues was observed
Precision
Intra-Assay Precision (Precision within an assay) CV% 6%
Inter-Assay Precision (Precision between assays) CV% 5%
Recovery for Rat Reg1A ELISA Kit (Colorimetric)
Recovery
average = 94% for serum; average = 96% for EDTA plasma; average = 93% for cell culture media
Linearity
Scientific Data Images for Rat Reg1A ELISA Kit (Colorimetric)
ELISA: Rat Reg1A ELISA Kit (Colorimetric) [NBP2-82146] -
ELISA: Rat Reg1A ELISA Kit (Colorimetric) [NBP2-82146] - Standard Curve ReferenceKit Contents for Rat Reg1A ELISA Kit (Colorimetric)
- Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Reg1A
Long Name
Regenerating Islet-derived 1A
Alternate Names
ICRF, PSP, PTP
Gene Symbol
REG1A
Additional Reg1A Products
Product Documents for Rat Reg1A ELISA Kit (Colorimetric)
Product Specific Notices for Rat Reg1A ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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