Rat Renin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-80264

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

9.375 pg/mL

Assay Range

15.625-1000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.13%

Inter-Assay Precision (Precision between assays) CV% 5.04%

Recovery for Rat Renin ELISA Kit (Colorimetric)

Recovery

88-110%

Linearity

Rat Renin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Renin ELISA Kit (Colorimetric)

Rat Renin ELISA Kit (Colorimetric)

ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] -

ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Standard Curve Reference

Kit Contents for Rat Renin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Renin

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.

Alternate Names

Angiotensinogenase, REN

Gene Symbol

REN

UniProt

P08424 (Rat)

Additional Renin Products

Product Documents for Rat Renin ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat Renin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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