Retinol Binding Protein RBP Antibody (SPM442)

Novus Biologicals | Catalog # NBP2-34362

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Goat, Monkey, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM442
View all Retinol Binding Protein RBP Primary Antibodies »

Product Specifications

Immunogen

Retinol binding protein purified from human plasma

Epitope

aa 74-182

Localization

Cytoplasmic

Specificity

Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This monoclonal antibody recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34410)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Retinol Binding Protein RBP Antibody (SPM442)

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Retinol Binding Protein RBP antibody (SPM442).

Applications for Retinol Binding Protein RBP Antibody (SPM442)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Retinol Binding Protein RBP

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Alternate Names

C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular

Gene Symbol

RBP1

UniProt

P02753, P09455 (Human), P50120 (Human)

Additional Retinol Binding Protein RBP Products

Product Documents for Retinol Binding Protein RBP Antibody (SPM442)

Certificate of Analysis

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Product Specific Notices for Retinol Binding Protein RBP Antibody (SPM442)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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