Retinol Binding Protein RBP Antibody

Novus Biologicals | Catalog # NB100-1494

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence VEGVVCKQVFKKVQ corresponding to C-Terminus according to NP_002890.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Retinol Binding Protein RBP Antibody

Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494]

Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494]

Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.
Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494]

Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494]

Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed U-2 OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494]

Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494]

Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Retinol Binding Protein RBP Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

0.5 - 3 ug/mL
Application Notes
IHC-P has been reported for previous lots.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Retinol Binding Protein RBP

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Alternate Names

C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular

Entrez Gene IDs

5947 (Human); 19659 (Mouse)

Gene Symbol

RBP1

UniProt

Additional Retinol Binding Protein RBP Products

Product Documents for Retinol Binding Protein RBP Antibody

Certificate of Analysis

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Product Specific Notices for Retinol Binding Protein RBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Retinol Binding Protein RBP Antibody

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Protocols

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