RFC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89341

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PAPAFSKAPGSAGHYELPWVEKYRPVKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAMLELNASND

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFC2 Antibody - BSA Free

Western Blot: RFC2 Antibody [NBP1-89341]

Western Blot: RFC2 Antibody [NBP1-89341]

Western Blot: RFC2 Antibody [NBP1-89341] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-89341]

Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-89341]

Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-89341] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341]

Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341]

Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341] - Staining of human vulva/anal skin shows strong nuclear positivity in squamous epithelial cells.
Western Blot: RFC2 Antibody [NBP1-89341]

Western Blot: RFC2 Antibody [NBP1-89341]

Western Blot: RFC2 Antibody [NBP1-89341] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
RFC2 Antibody

Immunohistochemistry: RFC2 Antibody [NBP1-89341] -

nbp1-89341_rabbit-polyclonal-rfc2-antibody-2552023151492.jpg
RFC2 Antibody

Immunohistochemistry: RFC2 Antibody [NBP1-89341] -

Immunohistochemistry: RFC2 Antibody [NBP1-89341] - Comparison of RFC2 expression between tumor & normal tissues (left, red represented tumor tissues while blue represented normal tissues) & corresponding immunohistochemistry images in normal tissues (middle) & tumor tissues (right). (A–F) The differential expression levels of RFC2 in glioblastoma (GBM) tissues (A), diffuse lower-grade gliomas (LGG) tissues (B), cervical cancer (CESC) tissues (C), colon adenocarcinoma (COAD) tissues (D), liver hepatocellular carcinoma (LIHC) tissues (E), lung squamous cell carcinoma (LUSC) tissues (F), & their corresponding normal tissues were evaluated based on GEPIA & HPA databases. (*P < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35210438), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RFC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFC2

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, MGC3665, replication factor C (activator 1) 2, 40kDa, replication factor C 2, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40replication factor C (activator 1) 2 (40kD)

Gene Symbol

RFC2

Additional RFC2 Products

Product Documents for RFC2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RFC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RFC2 Antibody - BSA Free

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Protocols

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FAQs

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