RFC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33083

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFC4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity determined by WB.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFC4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP1-33083]

Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP1-33083]

Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083]

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083]

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.
Western Blot: RFC4 Antibody [NBP1-33083]

Western Blot: RFC4 Antibody [NBP1-33083]

Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083]

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083]

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.
RFC4 Antibody

Western Blot: RFC4 Antibody [NBP1-33083] -

Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RFC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFC4

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, MGC27291, replication factor C (activator 1) 4, 37kDa, Replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37replication factor C (activator 1) 4 (37kD)

Gene Symbol

RFC4

Additional RFC4 Products

Product Documents for RFC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RFC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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