RFXANK Antibody (OTI3E7)

Novus Biologicals | Catalog # NBP2-01756

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E7
View all RFXANK Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RFXANK(NP_604389) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFXANK Antibody (OTI3E7)

Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756]

Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756]

Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.
Immunocytochemistry/ Immunofluorescence: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunocytochemistry/ Immunofluorescence: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunocytochemistry/Immunofluorescence: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RFXANK.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody.
Flow Cytometry: RFXANK Antibody (OTI3E7) [NBP2-01756]

Flow Cytometry: RFXANK Antibody (OTI3E7) [NBP2-01756]

Flow Cytometry: RFXANK Antibody (OTI3E7) [NBP2-01756] - Analysis of Jurkat cells, using anti-RFXANK antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RFXANK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFXANK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RFXANK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RFXANK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human Kidney tissue using anti-RFXANK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756]

Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human pancreas tissue using anti-RFXANK mouse monoclonal antibody.

Applications for RFXANK Antibody (OTI3E7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFXANK

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. [provided by RefSeq].

Alternate Names

ANKRA1Regulatory factor X subunit B, ankyrin repeat-containing regulatory factor X-associated protein, BLS, DNA-binding protein RFXANK, F14150_1, MGC138628, regulatory factor X-associated ankyrin-containing proteinAnkyrin repeat family A protein 1, RFXB, RFX-BRFX-Bdelta4

Entrez Gene IDs

8625 (Human)

Gene Symbol

RFXANK

Additional RFXANK Products

Product Documents for RFXANK Antibody (OTI3E7)

Certificate of Analysis

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Product Specific Notices for RFXANK Antibody (OTI3E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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