RGS5 Antibody (OTI1C1)

Novus Biologicals | Catalog # NBP2-00880

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C1
View all RGS5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RGS5 Antibody (OTI1C1)

Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880]

Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880]

RGS5-Antibody-OTI1C1-Immunohistochemistry-NBP2-00880-img0005.jpg
Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880]

Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880]

Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880]

Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880]

Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min)
Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]

Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody and then analyzed by flow cytometry.
RGS5 Antibody (OTI1C1)

Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] -

Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin & eosin (H&E), CK5/6, regulator of G‐protein signaling (RGS) 5, N‐cadherin, vimentin, & E‐cadherin in squamous cell carcinoma of the tongue in noninvasive & invasive portions. (a), (b), (c), (d), & (e) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 10× magnification. (f), (g), (h), (i), & (j) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 40× magnification. (k), (l), (m), (n), & (o) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 10× magnification. (p), (q), (r), (s), & (t) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 40× magnification Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31049219), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RGS5 Antibody (OTI1C1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RGS5

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, regulator of G-protein signaling 5, regulator of G-protein signalling 5

Entrez Gene IDs

8490 (Human)

Gene Symbol

RGS5

Additional RGS5 Products

Product Documents for RGS5 Antibody (OTI1C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RGS5 Antibody (OTI1C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RGS5 Antibody (OTI1C1)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for RGS5 Antibody (OTI1C1)

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  • Q: Could you tell us any information of epitope?

    A: About NBP2-00880 (RGS5 Antibody/1C1), it is a monoclonal mouse anti-human RGS5 antibody that was raised again the full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell. Therefore the narrow epitope could be anywhere in the full length protein. Unfortunately, our labs do not map the epitopes for any of our antibodies, including the monoclonal ones.

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