Rheb Antibody (7G7) - BSA Free

Novus Biologicals | Catalog # NBP3-26345

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7G7 expressed in HEK293

Format

BSA Free
View all Rheb Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Rheb [UniProt Q15382]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rheb Antibody (7G7) - BSA Free

Rheb Antibody (7G7)

Immunocytochemistry/Immunofluorescence: Rheb Antibody (7G7) [NBP3-26345] -

Immunocytochemistry/Immunofluorescence: Rheb Antibody (7G7) [NBP3-26345] - Staining of Hela Cells with Rheb Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Rheb Antibody (7G7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Rheb

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Gene Symbol

RHEB

Additional Rheb Products

Product Documents for Rheb Antibody (7G7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Rheb Antibody (7G7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Interferons

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Protocols

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