RhoA Antibody (1B3-4A10)

Novus Biologicals | Catalog # NBP2-22529

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1B3-4A10
View all RhoA Primary Antibodies »

Product Specifications

Immunogen

Full-length human recombinant RhoA protein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for RhoA Antibody (1B3-4A10)

Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529]

Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529]

Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a RhoA/C monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of human prostate carcinoma (right) compared with a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3%% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of mouse liver tissue (right) compared with a negative control without primary antibody (left).
Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529]

Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a RhoA/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.

Applications for RhoA Antibody (1B3-4A10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:50 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Immunoprecipitation

2 ug

Western Blot

1:500 - 1:1500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, Rho12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Gene Symbol

RHOA

Additional RhoA Products

Product Documents for RhoA Antibody (1B3-4A10)

Certificate of Analysis

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Product Specific Notices for RhoA Antibody (1B3-4A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RhoA Antibody (1B3-4A10)

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Protocols

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