RhoC Antibody (7X5I9)

Name: RhoC Antibody (7X5I9)
Brand: Novus Biologicals
SKU: NBP3-16333
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16333

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X5I9 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoC (P08134). MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoC Antibody (7X5I9)

Western Blot: RhoC Antibody (7X5I9) [NBP3-16333]

Western Blot: RhoC Antibody (7X5I9) [NBP3-16333] - Western blot analysis of extracts of various cell lines, using RhoC Rabbit mAb (NBP3-16333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333]

Immunocytochemistry/Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333] - Immunofluorescence analysis of NIH-3T3 cells using RhoC Rabbit mAb (NBP3-16333) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RhoC Antibody (7X5I9) [NBP3-16333]

Immunohistochemistry-Paraffin: RhoC Antibody (7X5I9) [NBP3-16333] - Immunohistochemistry of paraffin-embedded rat testis using RhoC Rabbit mAb (NBP3-16333) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333] -

Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333] - Immunofluorescence analysis of NIH-3T3 cells using RhoC Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Applications for RhoC Antibody (7X5I9)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoC

RhoC encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternate Names

ARH9ARHCRho cDNA clone 9, H9, MGC1448, MGC61427, oncogene RHO H9, ras homolog gene family, member C, RAS-related homolog 9, RhoC, rhoC GTPase, RHOH9, rho-related GTP-binding protein RhoC, small GTP binding protein RhoC

Gene Symbol

RHOC

Additional RhoC Products

Product Documents for RhoC Antibody (7X5I9)

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Certificate of Analysis

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Product Specific Notices for RhoC Antibody (7X5I9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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