RhoC Antibody

Novus Biologicals | Catalog # NBP2-20156

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RhoC Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RhoC. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (89%).

Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoC Antibody

Western Blot: RhoC Antibody [NBP2-20156]

Western Blot: RhoC Antibody [NBP2-20156]

Western Blot: RhoC Antibody [NBP2-20156] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RhoC Antibody [NBP2-20156]

Immunocytochemistry/ Immunofluorescence: RhoC Antibody [NBP2-20156]

Immunocytochemistry/Immunofluorescence: RhoC Antibody [NBP2-20156] - Sample: A431 cells were fixed in iced-cold MeOH for 5 min. Green: RHOC protein stained by RhoC antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: RhoC Antibody [NBP2-20156]

Immunohistochemistry-Paraffin: RhoC Antibody [NBP2-20156]

Immunohistochemistry-Paraffin: RhoC Antibody [NBP2-20156] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.
Western Blot: RhoC Antibody [NBP2-20156]

Western Blot: RhoC Antibody [NBP2-20156]

Western Blot: RhoC Antibody [NBP2-20156] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:3000.

Applications for RhoC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoC

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

ARH9ARHCRho cDNA clone 9, H9, MGC1448, MGC61427, oncogene RHO H9, ras homolog gene family, member C, RAS-related homolog 9, RhoC, rhoC GTPase, RHOH9, rho-related GTP-binding protein RhoC, small GTP binding protein RhoC

Gene Symbol

RHOC

UniProt

P08134 (Human)

Additional RhoC Products

Product Documents for RhoC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RhoC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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