RhoGDI Antibody (2G3) - BSA Free

Novus Biologicals | Catalog # NBP2-37472

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey, Primate

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G3

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human RhoGDI (AA: FULL(1-204)) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoGDI Antibody (2G3) - BSA Free

Western Blot: RhoGDI Antibody (2G3)BSA Free [NBP2-37472]

Western Blot: RhoGDI Antibody (2G3)BSA Free [NBP2-37472]

Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

Immunocytochemistry/ Immunofluorescence: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

Immunocytochemistry/Immunofluorescence: RhoGDI Antibody (2G3) [NBP2-37472] - Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

Flow Cytometry: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

Flow Cytometry: RhoGDI Antibody (2G3) [NBP2-37472] - Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).
Western Blot: RhoGDI Antibody (2G3)BSA Free [NBP2-37472]

Western Blot: RhoGDI Antibody (2G3)BSA Free [NBP2-37472]

Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein. (Expected MW is 48.7 kDa)
ELISA: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

ELISA: RhoGDI Antibody (2G3) - BSA Free [NBP2-37472]

ELISA: RhoGDI Antibody (2G3) [NBP2-37472] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for RhoGDI Antibody (2G3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoGDI

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Alternate Names

GDIA1, MGC117248, Rho GDI 1, Rho GDP dissociation inhibitor (GDI) alpha, rho GDP-dissociation inhibitor 1, RHOGDI, Rho-GDI alpha, RHOGDI-1

Gene Symbol

ARHGDIA

Additional RhoGDI Products

Product Documents for RhoGDI Antibody (2G3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RhoGDI Antibody (2G3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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