Ribophorin I Antibody (OTI3H3)

Novus Biologicals | Catalog # NBP2-01294

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H3
View all Ribophorin I Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ribophorin I Antibody (OTI3H3)

Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophorin I.
Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPN1 antibody, and then analyzed by flow cytometry.
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294]

Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.

Applications for Ribophorin I Antibody (OTI3H3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review.

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-01294 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ribophorin I

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq].

Alternate Names

DKFZp686B16177, dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, EC 2.4.1.119, oligosaccharyltransferase 1 homolog, OST1, ribophorin IRBPH1, Ribophorin-1, RPN-I

Entrez Gene IDs

6184 (Human)

Gene Symbol

RPN1

Additional Ribophorin I Products

Product Documents for Ribophorin I Antibody (OTI3H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ribophorin I Antibody (OTI3H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ribophorin I Antibody (OTI3H3)

Customer Reviews for Ribophorin I Antibody (OTI3H3) (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Ribophorin I Antibody (OTI3H3)
    Name: Teddy Chan
    Application: Western Blot
    Sample Tested: Brain Vascular Smooth Muscle Cell lysate
    Species: Human
    Verified Customer | Posted 02/08/2019
    Better reactivity when samples are not boiled
    Ribophorin I Antibody (OTI3H3) NBP2-01294
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human prostate cells tumor and non tumor
    Species: Human
    Verified Customer | Posted 07/01/2014
    expression of RPN1 on pca cell lines va normal
    Ribophorin I Antibody (OTI3H3) NBP2-01294

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