Rif1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1587

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rif1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2425 and the C-terminus (residue 2472) of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rif1 Antibody - BSA Free

Western Blot: Rif1 Antibody [NB100-1587]

Western Blot: Rif1 Antibody [NB100-1587]

Western Blot: Rif1 Antibody [NB100-1587] - Detection of Human Rif1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody NB100-1587 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Western Blot: Rif1 Antibody [NB100-1587]

Western Blot: Rif1 Antibody [NB100-1587]

Western Blot: Rif1 Antibody [NB100-1587] - Whole cell lysate from 293T cells. RIF1 was also immunoprecipitated using rabbit anti-RIF1 antibody NB100-1586.
Rif1 Antibody [NB100-1587] - Detection of human Rif1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody NB100-1587 used for IP at 6 ug per reaction. Rif1 was also immunoprecipitated by rabbit anti-Rif1 antibody NB100-1585. For blotting immunoprecipitated Rif1, NB100-1587 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Rif1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-5 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rif1

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human); 51869 (Mouse)

Gene Symbol

RIF1

UniProt

Q5UIP0 (Human)

Additional Rif1 Products

Product Documents for Rif1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rif1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rif1 Antibody - BSA Free

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Protocols

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