RIOK2 Antibody (OTI3E11)

Novus Biologicals | Catalog # NBP2-45960

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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E11
View all RIOK2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 277-552 of human RIOK2(NP_060813) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIOK2 Antibody (OTI3E11)

Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of extracts (35ug) from 9 different cell lines by usin g RIOK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of Adenocarcinoma of Human breast tissue.
Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Western Blot: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2.
Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of Human pancreas tissue.
Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunohistochemistry: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of Human bladder tissue.
Immunofluorescence: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunofluorescence: RIOK2 Antibody (OTI3E11) [NBP2-45960]

Immunofluorescence: RIOK2 Antibody (OTI3E11) [NBP2-45960] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RIOK2.
RIOK2 Antibody (OTI3E11)

RIOK2 Antibody (OTI3E11)[NBP2-45960]

RIOK2 Antibody (OTI3E11)[NBP2-45960]Simple Western� analysis of endogenous protein STK38L from HEK293T lysates (0.2 mg/mL) using STK38L Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for RIOK2 Antibody (OTI3E11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIOK2

RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.

Long Name

Serine/threonine-protein kinase RIO2

Alternate Names

RIO kinase 2, RIO2

Gene Symbol

RIOK2

Additional RIOK2 Products

Product Documents for RIOK2 Antibody (OTI3E11)

Certificate of Analysis

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Product Specific Notices for RIOK2 Antibody (OTI3E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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