![Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]](https://resources.rndsystems.com/images/products/RIP140-Antibody-Immunohistochemistry-Paraffin-NBP2-13674-img0007.jpg "Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]")
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: SSEAHLQQYSREHALKTQNANQAASERLAAMARLQENGQKDVGSYQLPKGMSSHLNGQARTSSSKLMASKSSATVFQNPMGIIPSSPKNAGYKNSLERNNIKQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RIP140 Antibody - BSA Free
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]](https://resources.rndsystems.com/images/products/RIP140-Antibody-Immunohistochemistry-Paraffin-NBP2-13674-img0004.jpg "Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]")
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]](https://resources.rndsystems.com/images/products/RIP140-Antibody-Immunohistochemistry-Paraffin-NBP2-13674-img0005.jpg "Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]")
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674] - Staining of human cerebellum shows moderate nuclear positivity in cells in granular layer.![Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]](https://resources.rndsystems.com/images/products/RIP140-Antibody-Immunohistochemistry-Paraffin-NBP2-13674-img0006.jpg "Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]")
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674]
Immunohistochemistry-Paraffin: RIP140 Antibody [NBP2-13674] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.Applications for RIP140 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. RIP140 antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using NBP2-13674 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RIP140
Alternate Names
10RIP140Receptor-interacting protein 140, FLJ77253, nuclear receptor interacting protein 1, nuclear receptor-interacting protein 1, receptor interacting protein 140
Gene Symbol
NRIP1
Additional RIP140 Products
Product Documents for RIP140 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RIP140 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for RIP140 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
Have you used RIP140 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: Lysate from HEK293 cellsSpecies: HumanVerified Customer | Posted 12/09/2014Immunoblot for NRIP1
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...