RNF183 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-74192

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RNF183 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to the N terminal of Rnf183. Immunizing peptide sequence LSLVTPARRRLLCPLCRQPTVLASGQPVTDLPTDTAMLTLLRLEPHHVIL. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for RNF183 Antibody - BSA Free

Western Blot: RNF183 Antibody [NBP1-74192]

Western Blot: RNF183 Antibody [NBP1-74192]

Western Blot: RNF183 Antibody [NBP1-74192] - Mouse Thymus Lysate 1ug/ml Gel Concentration 12%
RNF183 Antibody - BSA Free

Western Blot: RNF183 Antibody - BSA Free [NBP1-74192] -

ER alpha regulates RNF183 expression and increases its stability. The expression of endogenous RNF183 mRNA (A) and protein levels (B) in Ishikawa cells after transfecting with siER alpha or siNC. (C) Downregulated RNF183 protein level reduced by siER alpha was recovered based on MG132 treatment. (D) Depletion ER alpha weakens RNF183 stability. RNF183 protein level examined at indicated time after transfected with siER alpha 48h followed 100μg/mL cycloheximide treatment. Experiments were repeated in triplicates. Mean +/- S.D. (n = 3). *P<0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33324448), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RNF183 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF183

The function of this protein remains unknown.

Alternate Names

FLJ31197, MGC4734, ring finger protein 183

Gene Symbol

RNF183

UniProt

Q8QZS5 (Mouse)

Additional RNF183 Products

Product Documents for RNF183 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RNF183 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNF183 Antibody - BSA Free

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