RNF36 Antibody

Novus Biologicals | Catalog # NB100-1207

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RNF36 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GENKEPLHILHPQ corresponding to C-Terminus according to NP_892030.3, NP_542783.2.

Epitope

C-GENKEPLHILHPQ

Specificity

This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RNF36 Antibody

Western Blot: RNF36 Antibody [NB100-1207]

Western Blot: RNF36 Antibody [NB100-1207]

Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - Staining of paraffin embedded Human Cerebellum at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RNF36 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in mouse testis lysates (calculated MW of 57.3 kDa band according to mouse NP_536771.2). IHC-P: Human testis shows speckled staining in nuclei of spermatocytes and spermitids.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF36

The gene encoding this protein is specifically expressed in germ cells at round spermatid stages during spermatogenesis. The protein encoded by this gene contains an N-terminal RING finger motif, a B-box, and a C-terminal B-30.2-like domain. When overexpressed in COS-7 and HEK-293 cells, this protein was shown to induce cell apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

RFP-like domain-containing protein trimless, RING finger protein 36HSD34, RNF36, Trif, trimless, tripartite motif containing 69, tripartite motif protein 69, tripartite motif-containing 69, tripartite motif-containing protein 69

Entrez Gene IDs

140691 (Human)

Gene Symbol

TRIM69

UniProt

NP_892030.3 (Human)

Additional RNF36 Products

Product Documents for RNF36 Antibody

Certificate of Analysis

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Product Specific Notices for RNF36 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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