RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) - BSA Free

Novus Biologicals | Catalog # NBP2-76396

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-165-5F1

Format

BSA Free
View all RPA2 Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).

Modification

p Ser8, p Ser4

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) - BSA Free

Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of Human Phospho RPA32 (S4/S8) in NBF-fixed HeLa cells treated with EPE (right) or mock treated (left) by ICC-IF. Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Detection: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Immunoprecipitation: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of immunoprecipitates from HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396) and another rabbit anti-RPA32 antibody.

Applications for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunoprecipitation

6 ul/1.0 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: RPA2

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Gene Symbol

RPA2

Additional RPA2 Products

Product Documents for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) - BSA Free

Certificate of Analysis

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Product Specific Notices for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) - BSA Free

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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