RPA2 Antibody (OTI9A1)

Novus Biologicals | Catalog # NBP2-02182

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9A1
View all RPA2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA2 Antibody (OTI9A1)

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0021.jpg
Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.
Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.
Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.
Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
RPA2 Antibody (OTI9A1)

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] -

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 & IMR90 cells. (A) IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, & the pre-immune serum (PI) used as a control for the immunoprecipitation. (B) Western for RPA32-p-Thr21 in both HTC75 & IMR90Ajuba-depleted cells. A non-specific band is marked by a *. Total RPA32 levels are shown at the bottom. (C) Model for the role of Ajuba in repression of ATR. The “?” indicates that the interaction between Ajuba & RPA could be direct or indirect. See text for details. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23755068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RPA2 Antibody (OTI9A1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000

Reviewed Applications

Read 1 review rated 4 using NBP2-02182 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPA2

RPA2 is required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Entrez Gene IDs

6118 (Human)

Gene Symbol

RPA2

Additional RPA2 Products

Product Documents for RPA2 Antibody (OTI9A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RPA2 Antibody (OTI9A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPA2 Antibody (OTI9A1)

Customer Reviews for RPA2 Antibody (OTI9A1) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23755068
    Species: Human
    Verified Customer | Posted 12/29/2014

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Protocols

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