RPA70 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2204

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPA70 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human replication protein A1, 70kDa using the numbering given in entry NP_002936.1 (GeneID 6117).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA70 Antibody - BSA Free

Western Blot: RPA70 Antibody [NB100-2204]

Western Blot: RPA70 Antibody [NB100-2204]

Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204]

Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204]

Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No.used at a dilution of 1:1,000 (1 ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml (right). Detection: DAB
Western Blot: RPA70 Antibody [NB100-2204]

Western Blot: RPA70 Antibody [NB100-2204]

Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody NB100-2204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204]

Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204]

Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- RPA70 Lot2 used at a dilution of 1:1,000 (1 ug/ml) (left) and Lot3 used at a dilution of1:5,000 (0.2ug/ml) (right). Detection: DAB
Immunoprecipitation: RPA70 Antibody [NB100-2204]

Immunoprecipitation: RPA70 Antibody [NB100-2204]

Immunoprecipitation: RPA70 Antibody [NB100-2204] - Detection of human RPA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPA70 antibody NB100-2204 (lot NB100-2204-3) used for IP at 6 ug per reaction. RPA70 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2204-2). For blotting immunoprecipitated RPA70, NB100-2204 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RPA70 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPA70

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication. Alternate names for RPA70 include replication protein A 70 kDa DNA-binding subunit, RP-A, RF-A, Replication factor-A protein 1, single-stranded DNA-binding protein, p70, REPA1, RPA1, and HSSB.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Entrez Gene IDs

6117 (Human)

Gene Symbol

RPA1

UniProt

NP_002936.1 (Human)

Additional RPA70 Products

Product Documents for RPA70 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RPA70 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPA70 Antibody - BSA Free

Customer Reviews for RPA70 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review RPA70 Antibody - BSA Free and earn rewards!

Have you used RPA70 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies