RPA70 Antibody

Novus Biologicals | Catalog # NBP1-31382

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RPA70 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA70 Antibody

Immunocytochemistry/ Immunofluorescence: RPA70 Antibody [NBP1-31382]

Immunocytochemistry/ Immunofluorescence: RPA70 Antibody [NBP1-31382]

Immunocytochemistry/Immunofluorescence: RPA70 Antibody [NBP1-31382] - Analysis of HeLa, using NBP1-31382 at 1:200 dilution.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Lung papillary adenocarcinoma. RPA70 antibody [C1C3] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: RPA70 Antibody [NBP1-31382]

Western Blot: RPA70 Antibody [NBP1-31382]

Western Blot: RPA70 Antibody [NBP1-31382] - A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Cal27 xenograft, using RPA 70 kDa subunit antibody at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Mouse brain. RPA70 antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: RPA70 Antibody [NBP1-31382]

Immunoprecipitation: RPA70 Antibody [NBP1-31382]

Immunoprecipitation: RPA70 Antibody [NBP1-31382] - Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 ug of RPA70 antibody [C1C3].
RPA70 Antibody

Western Blot: RPA70 Antibody [NBP1-31382] -

Western Blot: RPA70 Antibody [NBP1-31382] - RPA70 antibody [C1C3] detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody [C1C3] (NBP1-31382) diluted at 1:1000.

Applications for RPA70 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPA70

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Gene Symbol

RPA1

Additional RPA70 Products

Product Documents for RPA70 Antibody

Certificate of Analysis

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Product Specific Notices for RPA70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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