Recombinant Human RPIA His Protein

Novus Biologicals | Catalog # NBP1-78855

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-311 of Human RPIA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD GSVNMREKPF C

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RPIA His Protein

SDS-PAGE: Recombinant Human RPIA His Protein [NBP1-78855]

SDS-PAGE: Recombinant Human RPIA His Protein [NBP1-78855]

SDS-Page: Recombinant Human RPIA Protein [NBP1-78855] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-78855
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 40% glycerol, 200 mM NaCl, 2 mM EDTA, 0.2 mM PMSF
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPIA

RPIA (Ribose-5-phosphate isomerase) belongs to the ribose 5-phosphate isomerase family. RPIA is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P). RPI exists as two distinct proteins forms, termed RPIA and RPIB. RPIA plays an essential role in the metabolism of plants and animals, as it is involved in the Calvin cycle which takes place in plants, and the pentose phosphate pathway which takes place in plants as well as animals. Recombinant human RPIA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 5.3.1.6, phosphoriboisomerase, ribose 5-phosphate epimerase, ribose 5-phosphate isomerase A, RPIribose-5-phosphate isomerase

Gene Symbol

RPIA

Additional RPIA Products

Product Documents for Recombinant Human RPIA His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human RPIA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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