RPL29 Antibody

Novus Biologicals | Catalog # NBP2-15103

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RPL29 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL29. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL29 Antibody

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-15103]

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-15103]

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-15103] - Paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.
RPL29 Antibody

Western Blot: RPL29 Antibody [NBP2-15103] -

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
12% SDS PAGE
NBP2-15103 diluted at 1:10000
RPL29 Antibody

Immunocytochemistry/ Immunofluorescence: RPL29 Antibody [NBP2-15103] -

RPL29 antibody detects RPL29 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL29 protein stained by RPL29 antibody (NBP2-15103) diluted at 1:500.
Blue: Hoechst 33343 staining.

Applications for RPL29 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPL29

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

60S ribosomal protein L29, Cell surface heparin-binding protein HIP, heparin/heparan sulfate-binding protein, heparin/heparan sulfate-interacting protein, HIPHUMRPL29, HP/HS-interacting protein, MGC88589, ribosomal protein L29

Gene Symbol

RPL29

UniProt

P47914 (Human)

Additional RPL29 Products

Product Documents for RPL29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RPL29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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