RPL32 Antibody (HL2331)
Novus Biologicals | Catalog # NBP3-48676
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2331
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Product Specifications
Immunogen
Full length of human RPL32 recombinant protein.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RPL32 Antibody (HL2331)
Western Blot: RPL32 Antibody (HL2331) [NBP3-48676] -
Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70�C
Immunohistochemistry: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70�C
Immunohistochemistry-Paraffin: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.
RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.
RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: RPL32 Antibody (HL2331) [NBP3-48676] -
Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: RPL32 Antibody (HL2331) [NBP3-48676] -
Whole Japanese medaka extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: RPL32 Antibody (HL2331) [NBP3-48676] -
Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein by immunofluorescent analysis.Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.
Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Immunocytochemistry/ Immunofluorescence: RPL32 Antibody (HL2331) [NBP3-48676] -
RPL32 antibody [HL2331] detects RPL32 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL32 stained by RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: RPL32 Antibody (HL2331) [NBP3-48676] -
Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2331] (NBP3-48676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for RPL32 Antibody (HL2331)
Application
Recommended Usage
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPL32
Alternate Names
M, ribosomal protein L32,60S ribosomal protein L32
Gene Symbol
RPL32
Additional RPL32 Products
Product Documents for RPL32 Antibody (HL2331)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPL32 Antibody (HL2331)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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