Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPL5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 247 and 297 of human Ribosomal Protein L5 using the numbering given in entry NP_000960.2 (GeneID 6125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL5 Antibody - BSA Free

Western Blot: RPL5 Antibody [NBP2-22285]

Western Blot: RPL5 Antibody [NBP2-22285]

Western Blot: RPL5 Antibody [NBP2-22285] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RPL5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.
Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB
Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB
Immunoprecipitation: RPL5 Antibody [NBP2-22285]

Immunoprecipitation: RPL5 Antibody [NBP2-22285]

Immunoprecipitation: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by western blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody NBP2-22285 used for IP at 3 ug per reaction. RPL5 was also immunoprecipitated by another rabbit anti-RPL5 antibody. For blotting immunoprecipitated RPL5, NBP2-22285 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RPL5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 5 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPL5

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Alternate Names

DBA6,60S ribosomal protein L5, MGC117339, ribosomal protein L5

Gene Symbol

RPL5

UniProt

NP_000960.2 (Human)

Additional RPL5 Products

Product Documents for RPL5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPL5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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