RPS15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20223

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Bovine (99%), Chicken (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS15. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPS15 Antibody - BSA Free

Immunohistochemistry-Paraffin: RPS15 Antibody [NBP2-20223]

Immunohistochemistry-Paraffin: RPS15 Antibody [NBP2-20223]

Immunohistochemistry-Paraffin: RPS15 Antibody [NBP2-20223] - Paraffin-embedded mouse liver. RPS15 antibody dilution: 1:500
RPS15 Antibody

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

Immunohistochemistry: RPS15 Antibody [NBP2-20223] - RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (NBP2-20223) dilution: 1:100.
RPS15 Antibody

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

Immunohistochemistry: RPS15 Antibody [NBP2-20223] - RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (NBP2-20223) dilution: 1:100.
RPS15 Antibody

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

Immunohistochemistry: RPS15 Antibody [NBP2-20223] - RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (NBP2-20223) dilution: 1:100.
RPS15 Antibody

Western Blot: RPS15 Antibody [NBP2-20223] -

Western Blot: RPS15 Antibody [NBP2-20223] - RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 ug U87-MG whole cell lysate/extract
B. 30 ug SK-N-SH whole cell lysate/extract
C. 30 ug IMR32 whole cell lysate/extract
D. 30 ug SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
RPS15 antibody (NBP2-20223) dilution: 1:1000
RPS15 Antibody

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

Immunohistochemistry: RPS15 Antibody [NBP2-20223] - RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (NBP2-20223) dilution: 1:100.
RPS15 Antibody - BSA Free

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

RPS15 antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
RPS15 antibody (NBP2-20223) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
RPS15 Antibody - BSA Free

Western Blot: RPS15 Antibody [NBP2-20223] -

RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 ug MCF-7 whole cell lysate/extract
B. 30 ug MDA-MB-231 whole cell lysate/extract
C. 30 ug IMR32 whole cell lysate/extract
D. 30 ug SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
RPS15 antibody (NBP2-20223) dilution: 1:1000
RPS15 Antibody - BSA Free

Immunohistochemistry: RPS15 Antibody [NBP2-20223] -

RPS15 antibody detects RPS15 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
RPS15 antibody (NBP2-20223) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for RPS15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPS15

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

40S ribosomal protein S15, homolog of rat insulinoma, MGC111130, ribosomal protein S15, RIG protein, RIGinsulinoma protein

Gene Symbol

RPS15

UniProt

Additional RPS15 Products

Product Documents for RPS15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RPS15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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