RPS20 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80804

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPS20 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25866880).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPS20 Antibody - BSA Free

Western Blot: RPS20 Antibody [NBP1-80804]

Western Blot: RPS20 Antibody [NBP1-80804]

Western Blot: RPS20 Antibody [NBP1-80804] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: RPS20 Antibody [NBP1-80804]

Immunocytochemistry/ Immunofluorescence: RPS20 Antibody [NBP1-80804]

Immunocytochemistry/Immunofluorescence: RPS20 Antibody [NBP1-80804] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human lymph node shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804]

Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human fallopian tube shows moderate positivity in glandular cells.

Applications for RPS20 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPS20

Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5' and 3' minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.

Alternate Names

40S ribosomal protein S20, FLJ27451, MGC102930, ribosomal protein S20

Entrez Gene IDs

6224 (Human); 67427 (Mouse); 122772 (Rat)

Gene Symbol

RPS20

UniProt

P60866 (Human)

Additional RPS20 Products

Product Documents for RPS20 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPS20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPS20 Antibody - BSA Free

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Protocols

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