RUNX1/CBFA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55213

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RUNX1/CBFA2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RUNX1/CBFA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody [NBP2-55213]

Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody [NBP2-55213]

Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody [NBP2-55213] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
RUNX1/CBFA2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RUNX1/CBFA2 Antibody - BSA Free [NBP2-55213]

Chromatin Immunoprecipitation-exo-Seq: RUNX1/CBFA2 Antibody - BSA Free [NBP2-55213]

ChIP-Exo-Seq composite graph for Anti-RUNX1 (NBP2-55213) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RUNX1/CBFA2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX1/CBFA2

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Runt-related Transcription Factor 1

Alternate Names

AML1, CBFA2

Gene Symbol

RUNX1

Additional RUNX1/CBFA2 Products

Product Documents for RUNX1/CBFA2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RUNX1/CBFA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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