RUVBL2 Antibody (OTI1A6)

Novus Biologicals | Catalog # NBP2-01764

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6
View all RUVBL2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUVBL2 Antibody (OTI1A6)

Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human tonsil using anti-RUVBL2 mouse monoclonal antibody.
Flow Cytometry: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Flow Cytometry: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Flow Cytometry: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody, and then analyzed by flow cytometry.
Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.
Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] Analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.
Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiRUVBL2 monoclonal antibody.(1:200)
Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (OTI1A6) [NBP2-01764]

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.
RUVBL2 Antibody (OTI1A6)

Western Blot: RUVBL2 Antibody (OTI1A6) [NBP2-01764] -

Western blot from DnaK immunoprecipitation comparing the five cancer cell lines. On the left side the Molecular Weight (MW) Marker is indicated. On the right side the name and MW of each validated protein are given. For each of the five cell types, three samples were loaded: the immunoprecipitated DnaK-transfected lysate with the antibody isotype control, the immunoprecipitated DnaK-transfected lysate with anti-V5 antibody and 2.5% load of the Dank-transfected lysate used for the immunoprecipitations. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36386669), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RUVBL2 Antibody (OTI1A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUVBL2

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].

Alternate Names

48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, EC 3.6.1, EC 3.6.4.12,48 kDa TBP-interacting protein, ECP51TIP49B, erythrocyte cytosolic protein, 51-KD, INO80 complex subunit J, INO80JTAP54-beta, Repressing pontin 52, REPTIN, Reptin 52, Reptin52, RuvB (E coli homolog)-like 2, ruvB-like 2, RuvB-like 2 (E. coli), RVB2, TBP-interacting protein, 48-KD, TIH2, TIP48ECP-51, TIP49b, TIP60-associated protein 54-beta

Entrez Gene IDs

10856 (Human)

Gene Symbol

RUVBL2

Additional RUVBL2 Products

Product Documents for RUVBL2 Antibody (OTI1A6)

Certificate of Analysis

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Product Specific Notices for RUVBL2 Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RUVBL2 Antibody (OTI1A6)

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Protocols

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